nebnext end prep master mix (New England Biolabs)
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nebnext end prep master mix
Nebnext End Prep Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1915 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebnext+ultra+ii+end+repair+da+tailing+module/NEBNext+Ultra+II+End+Repair%2FdA-Tailing+Module/pm42906542-1219-20-20
Average 99 stars, based on 1915 article reviews
Nebnext End Prep Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1915 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nebnext+ultra+ii+end+repair+da+tailing+module/NEBNext+Ultra+II+End+Repair%2FdA-Tailing+Module/pm42906542-1219-20-20
Average 99 stars, based on 1915 article reviews
nebnext end prep master mix - by Bioz Stars,
2026-11
99/100 stars
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Control:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel Article Snippet: .. Oxford Nanopore Technologies Native Barcoding Kit 24 V14 (SQK‐NBD114.24) or Native Barcoding Kit 96 V14 (SQK‐NBD114.96): Native Barcode plate (NB01‐24 or NB01‐96) DNA Control Sample (DCS) Native Adapter (NA) Sequencing Buffer (SB) Library Beads (LIB) Library Solution (LIS) Elution Buffer (EB) AMPure XP Beads (AXP) Long Fragment Buffer (LFB) Short Fragment Buffer (SFB) EDTA Flow Cell Flush (FCF) Flow Cell Tether (FCT) New England Biolabs (NEB) specific reagents for Oxford Nanopore Technologies: Sequencing:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel Article Snippet: .. Oxford Nanopore Technologies Native Barcoding Kit 24 V14 (SQK‐NBD114.24) or Native Barcoding Kit 96 V14 (SQK‐NBD114.96): Native Barcode plate (NB01‐24 or NB01‐96) DNA Control Sample (DCS) Native Adapter (NA) Sequencing Buffer (SB) Library Beads (LIB) Library Solution (LIS) Elution Buffer (EB) AMPure XP Beads (AXP) Long Fragment Buffer (LFB) Short Fragment Buffer (SFB) EDTA Flow Cell Flush (FCF) Flow Cell Tether (FCT) New England Biolabs (NEB) specific reagents for Oxford Nanopore Technologies: Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the Ligation:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel Article Snippet: .. Oxford Nanopore Technologies Native Barcoding Kit 24 V14 (SQK‐NBD114.24) or Native Barcoding Kit 96 V14 (SQK‐NBD114.96): Native Barcode plate (NB01‐24 or NB01‐96) DNA Control Sample (DCS) Native Adapter (NA) Sequencing Buffer (SB) Library Beads (LIB) Library Solution (LIS) Elution Buffer (EB) AMPure XP Beads (AXP) Long Fragment Buffer (LFB) Short Fragment Buffer (SFB) EDTA Flow Cell Flush (FCF) Flow Cell Tether (FCT) New England Biolabs (NEB) specific reagents for Oxford Nanopore Technologies: Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the Polymerase Chain Reaction:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel Article Snippet: .. Oxford Nanopore Technologies Native Barcoding Kit 24 V14 (SQK‐NBD114.24) or Native Barcoding Kit 96 V14 (SQK‐NBD114.96): Native Barcode plate (NB01‐24 or NB01‐96) DNA Control Sample (DCS) Native Adapter (NA) Sequencing Buffer (SB) Library Beads (LIB) Library Solution (LIS) Elution Buffer (EB) AMPure XP Beads (AXP) Long Fragment Buffer (LFB) Short Fragment Buffer (SFB) EDTA Flow Cell Flush (FCF) Flow Cell Tether (FCT) New England Biolabs (NEB) specific reagents for Oxford Nanopore Technologies: Gentle:Article Title: Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi‐Marker Amplicon Panel Article Snippet: .. Oxford Nanopore Technologies Native Barcoding Kit 24 V14 (SQK‐NBD114.24) or Native Barcoding Kit 96 V14 (SQK‐NBD114.96): Native Barcode plate (NB01‐24 or NB01‐96) DNA Control Sample (DCS) Native Adapter (NA) Sequencing Buffer (SB) Library Beads (LIB) Library Solution (LIS) Elution Buffer (EB) AMPure XP Beads (AXP) Long Fragment Buffer (LFB) Short Fragment Buffer (SFB) EDTA Flow Cell Flush (FCF) Flow Cell Tether (FCT) New England Biolabs (NEB) specific reagents for Oxford Nanopore Technologies: other:Article Title: Cell-free DNA methylome and fragmentome analysis for relapse monitoring of Ewing sarcoma Article Snippet: Amplification:Article Title: Development and evaluation of a real-time RT-PCR assay for Mammarenavirus guanaritoense virus detection Article Snippet: .. Amplicon (130 ng) was repaired using the Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the Whole-Genome Sequencing:Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the Quantitative RT-PCR:Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the Platinum:Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the DNA Library Preparation:Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the Purification:Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the Generated:Article Title: Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. Article Snippet: Additional swabs were collected aseptically from the chorioallantoic membrane (cases 2–5) and amnion (case 1) in transport medium (PrimeStore; ThermoFisher) for molecular testing. .. In preparation for whole-genome sequencing, the 8 influenza gene segments were amplified simultaneously in a single reaction using an RT-qPCR assay14–16 with the following universal primers: MBTuni-12 (0.1 μM), MBTuni-12.4 (0.1 μM), and MBTuni-13 (0.2 μM).18 A 25-μL reaction was used (Invitrogen Superscript III one-step RT-qPCR system, Platinum Taq DNA polymerase high fidelity; ThermoFisher), along with the following thermocycling reactions: 60 min at 42°C, 2 min at 94°C, then 5 cycles of 30 s at 94°C, 30 s at 44°C, 3 min at 68°C, followed by 31 cycles of 30 s at 94°C, 30 s at 57°C, 3 min at 68°C, and a final extension at 68°C for 7 min. For DNA library preparation, 500 ng of purified universal RT-qPCR amplicon from each sample was used as input for a library generated with the ligation sequencing kit SQK-LSK114 (Oxford Nanopore Technologies [ONT]).7 First, DNA was end-repaired using the |
